Membrane dehumidification process using defect free hollow fiber membrane. Projects to generate grams of monoclonal antibody have been successfully managed with this system, for example, in cell. Operating diagrams are presented that describe reactor performance as a function of important operating variables like oxygen concentration and the fiber packing density. The membranes modules are submerged in a biological tank or side streams tank, with the water being drawn. Cells suspended in tissue culture medium are inoculated into the extra.
Excimer laser modification was utilized to fabricate hollow fiber scaffolds allowing control over axonal outgrowth from neurons within a 3d space. The cells are nonembryonic stem, nongerm cells that can be characterized by one or more of the following. Wenten department of chemical engineering, itb, jl. It has the added advantage of having the highest surface area to volume ratio of all bioreactors. Evaluation of reagents used to coat the hollowfiber bioreactor membrane of the.
Development of hollow fiberbased bioreactor systems for. Use of hollow fiber systems for rapid and direct scale up of. These hollow fiber membranes are often bundled and housed within tubular polycarbonate shells to create hollow fiber bioreactor cartridges. Estimated membrane life of the current product should be greater than 10 years. The flns murine hybridoma cell line was simultaneously inoculated at 2 million cells per ml in a cl and the maximizer hollow fiber bioreactor system, a 21fold theoretical scale up over the cl. Alternative form of hollowfibre 2008, leda castilho. Abstractthe development of tissue engineering hollow fiber bioreactors hfb requires the.
Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. Dynamic simulation of bioreactors is a challenge for both the industrial and academic worlds. Jan 26, 2002 bioreactor design is a program which helps engineers and scientists to develop bioreactors for fermentation and cell cultivation. Membranes are semipermeable barriers and are found throughout the body to control the movement of nutrients and waste products in the various organs, for example blood vessel walls and the kidney. Aqp hffo2 is a cross flow membrane module containing aquaporin inside hollow fiber membranes. This book discusses the applications of biological kinetics and thermodynamics. Fouling control in activated sludge submerged hollow fiber. Hollow fiber membranes are ubiquitously used in industrial separations, especially the filtration of drinking water. Harvest large quantities of secreted protein with hollow. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. Tubular membranes were available for laboratory use as early as the 1920s and were first used in industrial applications in the 1960s. Slide the ends of the l s16 module connectors over the module ends and free side port.
A wide variety of hollow fiber bioreactor options are available to you, such as alcohol, milk. This manuscript describes the setup and operation of a hollow fiber bioreactor system for in vivolike mammalian tissue culture cite this article. A wide variety of hollow equipment options are available to you, there are 6,196 suppliers who sells hollow equipment on, mainly located in asia. The reason for this lies in the interesting features of this bioreactor type, including excellent mass transport properties. Biological cultures are most commonly performed in flasks, roller bottles or spinners. For adherent cell types, hfbs offer an in vivolike microenvironment as each fibre replicates a blood capillary and the mass transfer rate across the wall is independent from the shear stresses experienced by the cell. The 3d commercial bioreactor used with the quantum system contains about 11,500 hollow fibers, providing a 2. The wellformed dynamic membrane dm layer also called a secondary membrane can be used as a filter prior to the support material, and it can provide effective retention in both aerobic dm bioreactors dmbr and anaerobic 4 dm bioreactors andmbrs combined with several unique merits such as low membrane cost, high flux, and easy cleaning. Advantages of hollow fiber bioreactors from fibercell systems duration.
A strategy to determine operating parameters in tissue engineering. The shellandtube structure makes heat and mass transfer. The hollow fiber bioreactor disposable set is a highly efficient bioreactor configuration suitable for small scale biomass or protein production. Hollowfiber membrane an overview sciencedirect topics.
Jun 01, 2001 the bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Pdf hollow fiber bioreactors are the focus of scientific research aiming to mimic physiological vascular networks and. Ideal for ultrafiltration, diafiltration and microfiltration, singleuse spectrum hollow fiber filters for tangential flow filtration are packed in housings engineered to handle high pressures, with a choice of fiber chemistries and sizes from process development to manufacturing. Herein, electroassisted cntshfms served a dual function as the cathode and membrane filtration.
Bioreactor design cnet download free software, apps. Randallb school of biological resources and materials engineering, college of agriculture and life sciences, seoul national university, suwon 441744, korea. Hollow fiber membrane bioreactor hfmbr can shorten methane transfer pathway and improve methane transfer chen et al. Fibercell systems hollow fibre bioreactor for high density. Advantages of hollow fiber bioreactors from fibercell systems. Hollow fiber bioreactors for mammalian cell culture. This manuscript describes the setup and operation of a hollow fiber bioreactor system for in vivolike mammalian tissue culture.
Filtration principles, operation, and membrane fouling desalination 283 2011 39 contents lists available at sciencedirect desalination j o u r n a l h o m e p a g e. Cellmax duo hollow fiber cell culture system cellmax duo hollow fiber cell culture system cellmax, hollow fiber cell culture technology initiation of culture in the cellmax, cells are grown on and around a network of artificial capillaries encased in a cartridge shell. A hollow fiber bioreactor from fibercell systems is the ideal method for the in vitro production of 25mg on up to gram quantities of a monoclonal antibody. Application of submerged hollow fiber membrane in membrane. The design and mode of operation of a bioreactor depends on the production of organism. Biorec is an open source control software written for windows operating systems to provide data logging, process monitoring and control for pilot and laboratory scale bioflo series eppendorf ag, hamburggermany fermentorsbioreactors.
Incorporation of these scaffolds into a novel hollow fiber based bioreactor design will produce a device for high density neuronal tissue formation with axonal outgrowth in a 3d configuration. Hollow fiber bioreactors for in vivolike mammalian tissue. The main shortcoming of the stirred bioreactor, its mechanical agitation, is solved in other bioreactor designs. Review of hollow fiber and flat plate components what s on the horizon. The computeraided design cad files and all associated content posted to this website are created, uploaded, managed and owned by third party users. Organized into seven chapters, this book begins with an overview of the design of biological reactors that involves determining operating conditions, sizing the reactor, controlling.
Advantages and disadvantages of microporous membranes in a hollow fiber bioreactor for space applications by maria noel ruiz careri, b. Hollow fiber bioreactors are the focus of scientific research aiming to mimic physiological. Coculture of stromal and erythroleukemia cells in a perfused hollow fiber bioreactor system as an in vitro bone marrow model for myeloid leukemia. Involves designing vessel, headplate, temperature, ph, airoxygen, stirring control systems, and software to control these. Membrane life was determined by analysis of all zeeweed mbr installed in north american since their introduction in 1996. The top countries of suppliers are turkey, china, and pakistan, from which the percentage of hollow equipment supply is. Download pdf copy the hollow fiber bioreactordisposable set is a highly efficient bioreactor configuration suitable for small scale biomass or protein production. Membrane bioreactors can have flat sheet, hollow fibre, tubular or spiralwound membranes.
The activity of damo microorganisms directly affects the nitrogen removal rate of the damo and anammox coculture system. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the. Hollow fiber membrane life in membrane bioreactors mbr. Pdf criticality of flux and aeration for a hollow fiber. Unlike traditional cell culture methods, tflasks, roller bottles, and two compartment bioreactors, cellmax hollow fiber bioreactors provide a three dimensional space for in vitro cell culture. Application of submerged hollow fiber membrane in membrane bioreactors. There are different types of membranes available for use in mbrs. About 84% of these are water treatment, 14% are fermenting equipment, and 1% are reactors. Largescale production of lentiviral vector in a closed. Download our poster to learn how this novel singleuse technology can help you achieve successful cell culture scale. Mbr, membrane bioreactor, mbr module, flat sheet membrane. Widiasa department of chemical engineering, institut teknologi bandung, jalan ganesha 10 bandung, indonesia, 402 email. Pdf hollow fibre bioreactor for wastewater treatment. Design and operating criteria for hollow fiber bioreactors.
Hollow fiber membrane bioreactor affects microbial community. Production of exosomes in a hollow fiber bioreactor john j s cadwell, president and ceo, fibercell systems inc introduction exosomes are small lipid membrane vesicles 80120 nm of endocytic origin generated by fusion of cytoplasmic endosomal multivesicular bodies with the cell surface. Hollow fiber bioreactor technology for tissue engineering applications. Hollow fiber bioreactors for in vivolike mammalian tissue culture. Fibercell systems hollow fibre bioreactor for high density cell culture.
Cell expansion system hollowfiber bioreactor for the culture of mesenchymal. Production of exosomes in a hollow fiber bioreactor. The hollow fiber bioreactor and cell cocultivation. Fouling control in activated sludge submerged hollow fiber membrane bioreactors s. Simple and effective oxygen supply the ohio state university low energy requirement uniform distribution of energy bioreactor design. Hollow fibre membrane bioreactors hfb provide a novel approach towards tissue engineering applications in the field of regenerative medicine. Hollow fibre bioreactors show promise as cell expansion devices, owing to. Aquaporin inside hffo2 hollow fiber fo membrane module.
This manuscript describes in detail the setup and operation of a hollow fiber bioreactor system for the in vivolike culture of mammalian cells. The functional behavior of cells in culture can be improved by culturing in more in vivolike 3dimensional culture environments 1621. Hollow fibers are commonly used substrates for specialized bioreactor systems, with the ability of some hollow fiber cartridges to culture billions of anchoragedependent cells within a relatively low bioreactor volume. Hollow fiber membrane bioreactor for cod biodegradation of. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Hollow fiber bioreactors comparison to stirred tank introduction cells are typically cultured in the ecs due to better cell retention. Hollow fiber bioreactors for mammalian cell culture nature. Evaluation of reagents used to coat the hollowfiber bioreactor. This new layout has shown good results in terms of hepatic functions i. An introduction to modeling of bioreactors bengt carlsson dept of systems and control information technology uppsala university march 24, 2009 abstract this material is made for the course \modelling of dynamic systems. May 26, 2016 this manuscript describes in detail the setup and operation of a hollow fiber bioreactor system for the in vivolike culture of mammalian cells. This work describes a novel bioreactor system for the cultivation of animal, insect, and plant cells using wave agitation induced by a rocking motion.
As a module, hollow fiber membranes are packed together to form a bundle which is then placed in a shell and sealed at both ends. The fibercell hollow fiber bioreactor continuous 3d culture at high density to generate large. Mathematical modelling of cell layer growth in a hollow fibre bioreactor. A pump circulates ic medium from the ic reservoir, through a gas exchange cartridge, through the ics inside the bioreactor, and back to the ic reservoir. C3 has four hollow fiber bioreactor hfbrx systems to address a wide range of production applications. Ecologix mbr module systems replace the clarifier, sand filter and disinfection processes used in conventional activated sludge cas process by removing the suspended materials with ultrafiltration membrane. Nov 12, 2015 hollow fibre membrane bioreactor system. Hollow fiber reactors reduce cost, scaleup concerns by angelo depalma pharmaceutical manufacturers producing up to about 100 kgyr of therapeutic or diagnostic antibodies may soon replace roller bottles, ascites, stirred tanks, and airlift fermenters with hollow fiber bioreactors. Bioreactor free download as powerpoint presentation. Hollow fibre membrane bioreactors for tissue engineering. In the bubble column bioreactor, the mechanical impeller is exchanged with raising bubbles, which, in the case of an aerobic fermentation will anyhow be required and to the bene.
Us20120308531a1 expansion of stem cells in hollow fiber. Stem cells kdbio distributor for the fibercell systems. They provide extremely high surfacetovolume ratios, supporting large numbers of cells at. A technologically advanced highgrossfiltration rate hydrophilic polysulfone fiber with a molecular weight cut off mwco of 20kd is specified. Hollow fiber membrane bundles are widely used as liquidtoair contactors. Unlike other cell culture systems, such as spinners, hollow fiber bioreactors, and. Pdf hollow fiber bioreactor technology for tissue engineering. A better way to grow cells single page pdf download.
Membrane life is a significant component of operating costs. The hollow fiber bioreactor replicates the capillary system found in most tissues. How to optimize hollowfiber submerged membrane bioreactors. Bioreactor an apparatus usually jacketed cylindrical ss vessel for growing organisms such as bacteria, viruses, or yeast that are used in the production of pharmaceuticals, antibodies, or vaccines, or for the bioconversion of organic wastes under optimum conditions of gas air, oxygen, nitrogen. Membrane bioreactors an overview sciencedirect topics. Desirable properties of bioreactors bioreactor simplicity of design continuous operation w narrow distribution time large number of organisms per unit volume prof. Supported pvdf capillary fiber highly chlorine tolerant membrane high fluxlow pressure operation greatly reduced footprint as compared to conventional treatment hollow fiber membranes for membrane bioreactor applications in wastewater treatment worldwide sales and field service team proven engineering history membrane system design support. Highlights membrane bioreactors mbr have total installed capacity exceeding 5 million m3d. The hollow fiber bioreactor as a stromasupported, serum. Hollow fiber bioreactors have been in use for more than 30 years for the production of secreted proteins and antibodies from mammalian cells. Hollow fiber bioreactors introduction achieving high cell densities and reducing the costs to produce biologics are desirable but not always simple or inexpensive goals. The potential application of hollow fiber bioreactors to. Membrane bioreactor mbr technology, which is a combination of biologicalactivated sludge process and membrane filtration is widely spread, and accepted in recent years for the treatment of many types of wastewaters, while the conventional activated sludge cas process.
A 3d scaffoldfree microspheroid culture system and a 3d hollowfiber perfusion bioreactor. The bioreactors compact design allows a substantial amount of. A simple algorithm for solving the hollow fiber bioreactor design equations has been presented. The hollow fiber system was run in batch mode to mimic the method used for the cl with bd mab medium. Hollow fiber bioreactor technology for tissue engineering. C3s hollow fiber bioreactor systems are a solution to both goals. Disposable bioreactor for cell culture using waveinduced.
The hollow fiber bioreactor as a stromasupported, serum free ex vivo expansion platform for human umbilical cord blood cells. Referenced text membrane systems for wastewater treatment by water environment federation. Hollow fiber bioreactor, hollow fiber bioreactor suppliers. The hollowfiber configuration is most commonly used in. Highlights membrane bioreactor was used for treating high cod tapioca wastewater. Cells can either be seeded directly onto the outer surface of the fibres, or in a hydrogel throughout the ecs wung et al. Pdf hollow fibre membrane bioreactors for tissue engineering. In this study, we developed a biomimetic coculture platform using the hollow fiber bioreactor hfbr for ex vivo expansion of hscs. Advances in fiber construction and methods for oxygenation have the potential to bring the advantages of hollow fiber cell culture to biomanufacturing. A fibercell systems hollow fibre bioreactor enables the continuous culture of large numbers of cells at high density.
Hollow fiber membrane bioreactor for cod biodegradation of tapioca wastewater. This article is from biotechnology and bioengineering, volume 108. Beyond the large number of physical and biological phenomena to be considered and the wide range of scales involved, the central difficulty lies in the need to account for the dynamic behavior of suspended microorganisms. Jul 09, 2016 however, implementing smallscale hollow fiber bioreactors for culturing mammalian cells can save a lot of time and money, and is especially useful for harvesting large quantities of secreted protein. Enzymatic hollow fiber membrane bioreactor for penicilin. The development of tissue engineering hollow fiber bioreactors hfb. Novel anaerobic electrochemical membrane bioreactor with a. Hepatic differentiation of human ipscs in different 3d models. Hollow fiber reactors reduce cost, scaleup concerns. With an aquaporin inside coating on the lumen side, feed solution will be circulated in the lumen and draw solution will be circulated in the shell. Pdf hollow fibre membrane bioreactors hfb provide a novel approach towards tissue engineering. Hollow fiber bioreactors are the focus of scientific research aiming to mimic physiological vascular networks and engineer organs and tissues in vitro. Lecture notesbioreactor design and operation1 1 free download as powerpoint presentation.
To achieve large scale production of cultured meat it is necessary to select the bioreactor configuration that provides a suitable physiological environment. Hollow fiber bioreactors hfbrs have been employed in biotechnology for over 40 years, and their utility in the efficient and scalable production of exosomes has recently been reported. Enzymatic hollow fiber membrane bioreactor for penicilin hydrolysis i. The total surface area of a hollowfiber bioreactor varies in the range of 0.
Download fulltext pdf criticality of flux and aeration for a hollow fiber membrane bioreactor article pdf available in separation science and technology 457. Jun 12, 2012 figure 5 placental cells harvested from the hollow fiber bioreactor and placed in a t75 flask. Its a timescalable production process thats ideal for generating significant amounts of very concentrated evsexosomes, mabs or recombinant proteins. Slide the ends of the ls16 module connectors over the module ends and free side port. The terumo quantum cell expansion system is a hollow fiber bioreactor designed to expand cells to the 10 9 range in a manageable and compact culture vessel. Pdf optimizing t cell expansion in a hollowfiber bioreactor. New methods of largescale vector production have therefore been contemplated, and one of the most prominent concepts is a closed system hollow fiber bioreactor. A membrane bioreactor mbr is a combination of suspended growth activated sludge biological. A hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which. This agitation system provides good nutrient distribution, offbottom suspension, and excellent oxygen transfer without damaging fluid shear or gas bubbles.
Bioreactor design and operations a good bioreactor design should address improved productivity, validation of desired parameters towards obtaining consistent and higher quality products in a cost effective manner. Pdf how to optimize hollowfiber submerged membrane. Download fulltext pdf hollow fiber bioreactor technology for tissue engineering applications article pdf available in the international journal of artificial organs 391 february 2016 with. Bioreactor design fundamentals presents the development in the bioreactor field. Cellmax cellmax duo hollow fiber cell culture system.